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The Przewalski’s Horse Project

Photo: 5-day-old Przewalski’s horse clone at the ViaGen facility in Texas. Credit: Elizabeth Arellano Photography

The Przewalski’s horse (Equus przewalskii) is a critically endangered species that once ranged throughout Europe and Asia. Formerly extinct in the wild, today’s Przewalski’s horses are all descendants of just 12 individuals. Since 2018, Revive & Restore and its partners have worked to restore genetic diversity in the Przewalski’s horse through strategic conservation cloning.
Wild herd of Przewalski’s horses (Equus przewalskii)

Reintroduced Przewalski’s horses (Equus przewalskii) in Mongolia | Shutterstock

About the project

The Przewalski’s horse (pronounced “shuh-VAL-skees”) is the last truly wild horse species, diverging from the domestic horse over 500,000 years ago. Like many endangered species, the Przewalski’s horse is recovering from a severe historic bottleneck. All 2,000+ Przewalski’s horses today are descendants of just 12 individuals.

While ongoing reintroductions since the 1990s have established wild herds in China and Mongolia, restoring genetic variation is essential to ensure the species’ survival in the future.

In 2018, Revive & Restore, the San Diego Zoo Wildlife Alliance, and ViaGen Pets and Equine launched a collaboration to clone the world’s first Przewalski’s horse from a cell line that had been cryopreserved since 1980 at the San Diego Zoo Wildlife Alliance Frozen Zoo. The foal, named Kurt, was born on August 6, 2020 and carries precious genetic variation lost from the living gene pool.

On Feb. 17, 2023, a second cloned Przewalski’s horse was born from the same cell line. Kurt and the new foal are genetic twins that may become the first cloned animals to restore lost genetic variation to their species.

Meet the New Foal, born in 2023

Meet Kurt, born in 2020

It is an enormously hopeful, unprecedented step to have the capacity to restore genetic diversity. Kurt’s birth was a major milestone for Przewalski’s horse conservation. His success will serve as a model for saving endangered wildlife through the use of cloning.”

Oliver Ryder

Director of Conservation Genetics, San Diego Zoo Wildlife Alliance

OVERCOMING A GENETIC BOTTLENECK

Genetic rescue is a mitigation strategy to reduce the negative impacts of inbreeding by introducing new individuals. Since there were no unrelated Przewalski’s horses available, we cloned Kurt from a genetically diverse cell line that had been cryopreserved in 1980.

Watch the video: 3-month-old Kurt jumps and bucks while his surrogate mother stands by. (Courtesy Timber Creek Veterinary)