Nurturing embryonic development, Guojun Sheng, Kumamoto University



Deadline to submit a final proposal: May 1, 2022

Powerful new biotechnologies hold promise to revolutionize the way species are protected and restored. Biotechnologies developed for mammals for biomedical research and agricultural purposes can be readily modified for use in endangered mammals. Several pioneering efforts are already underway, applying cloning and developing stem cell embryogenesis for endangered mammals. However, biotechnologies developed in domestic chickens are in need of optimization and further innovation to be applicable to endangered birds. The Biotechnology for Bird Conservation Program aims to develop new biotechnology tools for use in avian species, potentially forging completely new transformative technologies.


The Biotechnology for Bird Conservation Program will accept proposals across four program tracks, as described below. Proposals should address a single program track. Teams wishing to address multiple program tracks are instructed to submit a separate proposal for each. The deadline to submit final proposals is May 1, 2022.

To advance germ-line transmission technologies towards the above listed capabilities, in the first two program tracks we are calling for proposals that address the following topics:

  1. Production of non-chicken birds via biparental germ-line transmission of cultured PGCs.
  2. Production of birds via biparental germ-line transmission of induced primordial germ cells.
  3. Efficient in vivo gene-editing of bird germ-lines.
  4. Novel innovation and/or adaptation of technologies from outside the field of avian systems. This program track will support multi-disciplinary projects that attempt to leverage advances in biomedical fields.

For scientists planning to apply, please download and review the detailed proposal guidelines and criteria and use these proposal and budget templates.

Selection Criteria

Proposals will be evaluated according to the following selection criteria: 

  • Technical Merit
    • How likely is it that the proposed approach will be successful?
    • What are the greatest technical risks and can they be mitigated?
  • Potential for Impact
    • If the project is successful, how will it enable genetic rescue of wild species?
    • Is there a plan to transition to proofs-of-concept with wild species?
  • Budget and Realistic Schedule
    • Are the budgets and timeline realistic for meeting the goals of the project?

Funding Preferences

Funding preference will be given to proposals with the following characteristics: 

  • Non-technical challenges and mitigations addressed: issues such as community acceptance and regulatory requirements should be carefully considered and planned for. 
  • Availability of matching funds: other funding may include government agencies, universities, or private sources. 
  • Revive & Restore is happy to support indirect costs up to 10 percent of the total project budget.

How to Apply

For scientists planning to apply, please download and review the detailed proposal guidelines and criteria and use these proposal and budget templates. We request that principal investigators (PI) send a letter of intent indicating which program track their proposed idea falls into and a 300 word summary of the idea at their earliest convenience.

Send letters of intent and final proposals to the Program Manager, Ben Novak (ben(at), cc’ing Revive & Restore’s Operations Manager, Marmee Manack, (marmee(at) In the email subject line write “BBC Letter of Intent: PI Name”. In the subject line for final proposals write “BBC Proposal: PI Name”